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avidin biotin complex kit  (Vector Laboratories)


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    Structured Review

    Vector Laboratories avidin biotin complex kit
    Avidin Biotin Complex Kit, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 12720 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/avidin+biotin+peroxidase+complex+kit/VECTASTAIN+Elite+ABC+HRP+Kit+(Peroxidase%2C+Standard)/pmc13122667-89-14-17
    Average 99 stars, based on 12720 article reviews
    avidin biotin complex kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Avidin-Biotin Assay:

    Article Title: Low-dose metformin requires brain Rap1 for its antidiabetic action
    Article Snippet: The sections were then incubated with c-Fos antibody (1:1000; Synaptic Systems, 226003) or GFP antibody (1:1000; Aves Labs, NC9510598) in 3% normal donkey serum in PBT with 0.02% sodium azide for 48 to 72 hours. .. They were then treated with biotinylated secondary antibody against rabbit immunoglobulin G (1:1000; Vector Laboratories, BA-1000) or the avidin-biotin-peroxidase complex kit (1:1000; Vector Laboratories, VECTASTAIN Elite ABC kit). .. Immunoreactivities were visualized with fluorescence-conjugated streptavidin (1:500; Life Technologies, Thermo Fisher Scientific).

    Article Title: Targeting of specific neuronal types in the non-human primate brain by using a murine CD25-specific recombinant immunotoxin.
    Article Snippet: The sections were then incubated with an anti-mouse IgG secondary antibody conjugated either to biotin (goat, 1:500; Jackson ImmunoResearch Laboratories, Cat#715-065-151) or to Cy3 (goat, 1:500; Jackson ImmunoResearch Laboratories, Cat#715-165-150) for 2 h at room temperature. .. Biotinylated signals were visualized with an avidin-biotin-peroxidase complex kit (Vector Laboratories, CatPK-6101). ..

    Article Title: Successful Treatment of an Acinar Pancreatic Carcinoma in an Inland Bearded Dragon ( Pogona vitticeps ): A Case Report
    Article Snippet: .. Signal enhancement was achieved with the avidin−biotin−peroxidase complex kit (VECTASTAIN ® Elite ® ABC-Kit, Vector Laboratories, Inc., Burlingame, CA, USA). ..

    Article Title: Distinct roles of two thalamostriatal systems in learning processes of visual discrimination in common marmosets
    Article Snippet: Then the sections were incubated with species-specific IgG antibodies conjugated to biotin (for rabbit, 1:500, #711-065-152; Jackson ImmunoResearch Labs, West Grove, PA, USA or for goat, 1:500, #711-065-147; Jackson ImmunoResearch Labs), Cy3 (for guinea pig, 1:500, #706-165-148; Jackson ImmunoResearch Labs), Alexa 488 (for rabbit, 1:500, #A-21206; Thermo Fisher Scientific, Waltham, MA, USA) or Alexa 647 (for mouse, 1:500, #A-31571; Thermo Fisher Scientific) for 2 h at room temperature. .. The sections were treated with an avidin-biotin-peroxidase complex kit (VECTASTAIN Elite ABC kit; Vector Laboratories Inc., Newark, CA, USA). .. To visualize gene expression, sections were reacted with 0.05 M Tris-HCl buffer (pH 7.6) containing 0.05% 3,3’-diaminobenzidine tetrahydrochloride (DAB; DOJINDO, Kumamoto, Japan) and 0.3% H 2 O 2 (FUJIFILM WAKO Pure Chemical, Osaka, Japan) for 5–10 min.

    Article Title: Symptomatic treatment by a BBB-permeable AAV engineered to restore TDP-43 function slows motor neuron disease and prevents paralysis
    Article Snippet: Then, the sections were washed three times with 1X PBS containing 1% Tween-20 (PBST) followed by incubation in biotinylated secondary antibodies (Anti-Mouse IgG, Ref#BP-9200 and Anti-Rabbit IgG, Ref#BP-9100, Vector laboratories) diluted in blocking buffer at room temperature for 1 hour. .. Sections were washed three times with PBS-T and incubated with avidin-biotin peroxidase complex kit (Ref#PK-7100, Vectastatin ABC Reagent, Vector Laboratories) for 1 hour. ..

    Article Title: Low-dose metformin requires brain Rap1 for its antidiabetic action.
    Article Snippet: The sections were then incubated with c- Fos antibody (1:1000; Synaptic Systems, 226003) or GFP antibody (1:1000; Aves Labs, NC9510598) in 3% normal donkey serum in PBT with 0.02% sodium azide for 48 to 72 hours. .. They were then treated with biotinylated secondary antibody against rabbit immunoglobulin G (1:1000; Vector Laboratories, BA- 1000) or the avidin- biotin- peroxidase complex kit (1:1000; Vector Laboratories, VECTASTAIN Elite ABC kit). .. Immunoreactivities were visualized with fluorescence- conjugated streptavidin (1:500; Life Technologies, Thermo Fisher Scientific).

    Article Title: Distinct roles of two thalamostriatal systems in visually triggered decision-making in common marmosets
    Article Snippet: Then the sections were incubated with species-specific IgG antibodies conjugated to biotin (for rabbit, 1:500, #711-065-152; Jackson ImmunoResearch Labs, West Grove, PA, USA or for goat, 1:500, #711-065-147; Jackson ImmunoResearch Labs), Cy3 (for guinea pig, 1:500, #706-165-148; Jackson ImmunoResearch Labs), Alexa 488 (for rabbit, 1:500, #A-21206; Thermo Fisher Scientific, Waltham, MA, USA) or Alexa 647 (for mouse, 1:500, #A-31571; Thermo Fisher Scientific) for 2 h at room temperature. .. The sections were treated with an avidin-biotin-peroxidase complex kit (VECTASTAIN Elite ABC kit; Vector Laboratories Inc., Newark, CA, USA). .. To visualize gene expression, sections were reacted with 0.05 M Tris-HCl buffer (pH 7.6) containing 0.05% 3,3′-diaminobenzidine tetrahydrochloride (DAB; DOJINDO, Kumamoto, Japan) and 0.3% H 2 O 2 (FUJIFILM WAKO Pure Chemical, Osaka, Japan) for 5–10 min.

    Article Title: Prenatal ethanol and cannabis exposure have sex- and region-specific effects on somatostatin and neuropeptide Y interneurons in the rat hippocampus.
    Article Snippet: Following this was a 2- h 2°C incubation in a 1:500 dilution of biotinylated goat α- rabbit (GenTex GTX71935) at room temperature in 3% NGS and 0.5% PBST. .. An avidin- biotin- peroxidase complex kit (Vector Labs, PK6100; MJS Biolynx, CA) was used according to the supplied instructions. .. After the incubation, brain tissue was washed three times for 10 min in PBS and then developed in 3,3′- Diaminobenzidine (Vector Labs, SK4100; MJS Biolynx, CA).

    Incubation:

    Article Title: Symptomatic treatment by a BBB-permeable AAV engineered to restore TDP-43 function slows motor neuron disease and prevents paralysis
    Article Snippet: Then, the sections were washed three times with 1X PBS containing 1% Tween-20 (PBST) followed by incubation in biotinylated secondary antibodies (Anti-Mouse IgG, Ref#BP-9200 and Anti-Rabbit IgG, Ref#BP-9100, Vector laboratories) diluted in blocking buffer at room temperature for 1 hour. .. Sections were washed three times with PBS-T and incubated with avidin-biotin peroxidase complex kit (Ref#PK-7100, Vectastatin ABC Reagent, Vector Laboratories) for 1 hour. ..



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